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Image Search Results
Journal: iScience
Article Title: Pseudouridine synthase 1 regulates erythropoiesis via transfer RNAs pseudouridylation and cytoplasmic translation
doi: 10.1016/j.isci.2024.109265
Figure Lengend Snippet:
Article Snippet: Anti-PUS1 (A-4) antibody (Santa Cruz biotechnology, sc-390043), Anti-Ndufs1 antibody (abcam, ab169540), Anti-Ndufs2 antibody (abcam, ab192022), Anti-GAPDH antibody (Cell Signaling Technology, 2118), Anti-Hemoglobin β/γ/δ/ε antibody (Santa Cruz biotechnology, sc-390668), Anti-ND3 antibody (abclonal, A17969),
Techniques: Recombinant, Virus, SYBR Green Assay, Cell Culture, Bicinchoninic Acid Protein Assay, Isolation, Iron Assay, Activity Assay, Dehydrogenase Assay, Cytochrome c Oxidase Assay, Viability Assay, RNA Sequencing Assay, Transgenic Assay, Primer Extension Assay, Software
Journal: Translational Psychiatry
Article Title: Oxytocin attenuates hypothalamic injury-induced cognitive dysfunction by inhibiting hippocampal ERK signaling and Aβ deposition
doi: 10.1038/s41398-024-02930-y
Figure Lengend Snippet: A results of GSEA analysis show upregulation of ERK signaling pathway in the PEL group (n = 6) compared to the Sham group (n = 6). Immunoblotting confirmed the elevated expression of p-ERK in hippocampal tissues of mice after PEL surgery ( B , C F (3.000, 12.18) = 109.2, p < 0.01). Immunoblotting confirmed elevated Aβ expression in hippocampal tissues of mice after PEL surgery, and OXT reduced Aβ expression ( B , D Kruskal–Wallis statistic=17.95, p < 0.01). Trajectory diagram of PEL group (n = 6), U0126 supplemented group (n = 6) and TBHQ group (n = 6) mice in the MWM place navigation experiment ( E ). Quantitative analysis of latency in the MWM place navigation experiment ( F ) (F (2, 69) = 22.05, p < 0.01). Trajectory diagrams of PEL group (n = 6), supplemented U0126 group (n = 6), and TBHQ group mice (n = 6) in the MWM spatial exploration experiment. The shaded part is the area around the platform, and the activity time of mice in the shaded part is calculated. G Quantitative analysis of activity time around the platform in the MWM spatial exploration experiment ( H ). (F (2, 15) = 16.57 p < 0.01). Data were analyzed by 1-way ANOVA followed by LSD multiple comparisons test followed by LSD multiple comparisons test ( C , H ), Kruskal–Wallis test ( D ) and two-way ANOVA ( F ). Data are expressed as mean ± SEM. Compared to Sham, ** P < 0.01. PEL, pituitary stalk electrical damage; GO gene ontology; GSEA gene set enrichment analysis, MWM Morris Water Maze.
Article Snippet: The following primary antibodies were used: rabbit anti-AVP (AB1565; Millipore), rabbit anti-OXT (ab212193; Abcam, rabbit anti-ERK (51068-1-AP; Proteintech), rabbit anti-p-ERK (28733-1-AP; Proteintech), rabbit anti-OXTR (23045-1-AP; Proteintech),
Techniques: Western Blot, Expressing, Activity Assay
Journal: iScience
Article Title: Pseudouridine synthase 1 regulates erythropoiesis via transfer RNAs pseudouridylation and cytoplasmic translation
doi: 10.1016/j.isci.2024.109265
Figure Lengend Snippet:
Article Snippet: Anti-PUS1 (A-4) antibody (Santa Cruz biotechnology, sc-390043), Anti-Ndufs1 antibody (abcam, ab169540), Anti-Ndufs2 antibody (abcam, ab192022), Anti-GAPDH antibody (Cell Signaling Technology, 2118), Anti-Hemoglobin β/γ/δ/ε antibody (Santa Cruz biotechnology, sc-390668), Anti-ND3 antibody (abclonal, A17969), Anti-ND6 antibody (abclonal, A17991),
Techniques: Recombinant, Virus, SYBR Green Assay, Cell Culture, Bicinchoninic Acid Protein Assay, Isolation, Iron Assay, Activity Assay, Dehydrogenase Assay, Cytochrome c Oxidase Assay, Viability Assay, RNA Sequencing Assay, Transgenic Assay, Primer Extension Assay, Software
Journal: iScience
Article Title: Pseudouridine synthase 1 regulates erythropoiesis via transfer RNAs pseudouridylation and cytoplasmic translation
doi: 10.1016/j.isci.2024.109265
Figure Lengend Snippet:
Article Snippet: Anti-PUS1 (A-4) antibody (Santa Cruz biotechnology, sc-390043), Anti-Ndufs1 antibody (abcam, ab169540), Anti-Ndufs2 antibody (abcam, ab192022), Anti-GAPDH antibody (Cell Signaling Technology, 2118), Anti-Hemoglobin β/γ/δ/ε antibody (Santa Cruz biotechnology, sc-390668),
Techniques: Recombinant, Virus, SYBR Green Assay, Cell Culture, Bicinchoninic Acid Protein Assay, Isolation, Iron Assay, Activity Assay, Dehydrogenase Assay, Cytochrome c Oxidase Assay, Viability Assay, RNA Sequencing Assay, Transgenic Assay, Primer Extension Assay, Software
Journal: The FASEB Journal
Article Title: COL6A1 , LAPTM5 , and ZFAND2A as Crucial Biomolecules Driving Immunoregulation in Human Nucleus Pulposus Degeneration
doi: 10.1096/fj.202500320R
Figure Lengend Snippet: MR analysis of genes associated with disc degeneration using integrated eQTL and FinnGen datasets. (A–H) Causal relationships between the eQTLs of individual genes (ATP13A3, BMP2, CD83, COL6A1, HLA‐DQA1, LAPTM5, NPC2, and ZFAND2A) and the risk of disc degeneration. (I–M) Leave‐one‐out analysis was performed separately for the MR studies of CD83, COL6A1, LAPTM5, NPC2, and ZFAND2A.
Article Snippet: The primary antibodies included CD83 (AF5233, Affinity), COL6A1 (17023–1‐AP, Proteintech), LAPTM5 (A17995, ABclonal),
Techniques:
Journal: The FASEB Journal
Article Title: COL6A1 , LAPTM5 , and ZFAND2A as Crucial Biomolecules Driving Immunoregulation in Human Nucleus Pulposus Degeneration
doi: 10.1096/fj.202500320R
Figure Lengend Snippet: Expression of key genes in NP tissues. (A–J) Western blotting and immunohistochemical staining show the protein levels of CD83, COL6A1, LAPTM5, NPC2, and ZFAND2A in relatively healthy and degenerative NP tissues.
Article Snippet: The primary antibodies included CD83 (AF5233, Affinity), COL6A1 (17023–1‐AP, Proteintech), LAPTM5 (A17995, ABclonal),
Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining